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Mixed or ambiguous sequence probes
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"2′-fluoro-modified pyrimidines enhance affinity of RNA oligonucleotides to HIV-1 reverse transcriptase
Excellent coupling efficiency and yield
DMT-2'-O-Me-A(Bz)-CE Phosphoramidite RNA (2'-O-TBDMS) Phosphoramidites Mixed or ambiguous sequence probesDMT 2' O Me A(Bz) CE Phosphoramidite is a 2' OMe phosphoramidite monomer used for solid phase oligonucleotide synthesis. It contains a 5' dimethoxytrityl (DMT) protecting group for 5' hydroxyl protection, a benzoyl (Bz) base protecting group on adenine, a cyanoethyl (CE) protecting group on phosphite, and a 2' O methyl (2' OMe) ribose modification The 2' OMe modification improves duplex stability, reduces immunogenicity, and significantly increases
